rabbit anti slc12a2  (Proteintech)

Journal: Frontiers in Microbiology

Article Title: High casein concentration induces diarrhea through mTOR signal pathway inhibition in post-weaning piglets

doi: 10.3389/fmicb.2024.1430511

Figure Lengend Snippet: Primers used for real-time quantitative PCR.

Article Snippet: The antibodies used for protein quantification in our study are as follows: beta-actin (60008-1, 1:5,000, Proteintech), RPS60KB1 (A16968, 1:1,000 ABclonal), SLC9A3 (AB2756978, 1:1,000 ABclonal), SLC12A2 (8351S, 1:2,000 CST), SLC26A3 (5642S, 1,1,000 CST), SLC26A6 (A10323, 1:1,000 ABclonal), and SLC5A1 (A13164, 1:1,000 ABclonal), along with the Horseradish Peroxidase (HRP)-conjugated secondary antibodies.

Techniques:

Journal: Frontiers in Microbiology

Article Title: High casein concentration induces diarrhea through mTOR signal pathway inhibition in post-weaning piglets

doi: 10.3389/fmicb.2024.1430511

Figure Lengend Snippet: Effect of different protein level diets on the relative gene expression of ion transporter carriers of post-weaning piglets. The relative expression of (A,F) SLC5A1, (B,G) SLC9A3, (C,H) SLC12A2, (D,I) of SLC26A3, and (E,J) SLC26A6. Values are expressed as mean ± SEM, n = 7. * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, p > 0.05.

Article Snippet: The antibodies used for protein quantification in our study are as follows: beta-actin (60008-1, 1:5,000, Proteintech), RPS60KB1 (A16968, 1:1,000 ABclonal), SLC9A3 (AB2756978, 1:1,000 ABclonal), SLC12A2 (8351S, 1:2,000 CST), SLC26A3 (5642S, 1,1,000 CST), SLC26A6 (A10323, 1:1,000 ABclonal), and SLC5A1 (A13164, 1:1,000 ABclonal), along with the Horseradish Peroxidase (HRP)-conjugated secondary antibodies.

Techniques: Gene Expression, Expressing

Journal: BMC Medicine

Article Title: Loss of KCNJ10 protein expression abolishes endocochlear potential and causes deafness in Pendred syndrome mouse model

doi: 10.1186/1741-7015-2-30

Figure Lengend Snippet: Protein localization of KCNQ1, KCNE1, SLC12A2 and GJB2 in the cochlear lateral wall of Slc26a4 +/+ and Slc26a4 -/- mice. a-f: bars: 10 μm. SMC, strial marginal cells; SV, stria vascularis; BC, basal cells; SL, spiral ligament.

Article Snippet: Slides were incubated overnight at 4°C with primary antibodies in PBS-TX with 1–3% BSA [rabbit anti-pendrin, 1:500 (h766–780); rat anti-ZO-1, 1:100 (Chemicon, Temecula, CA); goat anti-KCNQ1, 1:200 (C20, Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-KCNE1, 1:200 (Alomone, Jerusalem, Israel); rabbit anti-KCNJ10, 1:300 (Alomone); rabbit anti-SLC12A2, 1:100 (Chemicon); and rabbit anti-connexin 26, 1:100 (Zymed, San Francisco, CA)].

Techniques:

Journal: PLoS Biology

Article Title: Early uneven ear input induces long-lasting differences in left–right motor function

doi: 10.1371/journal.pbio.2002988

Figure Lengend Snippet: (A) Thirty-second trace of an Slc12a2 K842 * /K842 * and control mouse. Turns: blue, CC; red, C; green, none. (B) The majority of mutant mice have a bias in the C or CC direction. Slc12a2 K842 * /K842 *, C: n = 22; CC: n = 17; NP: n = 3. Foxg1 Cre/+ ; Slc12a2 fx/fx , C: n = 11; CC: n = 10. BAC 316.23, C: n = 3; CC: n = 5; NP: n = 1. Pax2-Cre;Tbx1 fx/fx , C: n = 4; mean ± SEM. See also . (C) The directional preference is stable over time. Mean percent of clockwise circles: Slc12a2 K842 * /K842 * C turners, day 1 versus 5, p > 0.9999; CC turners, day 1 versus 5, p > 0.9999; Foxg1 Cre/+ ; Slc12a2 fx/fx C turners, day 1 versus 31, p > 0.9999; CC turners, day 1 versus 31, p = 0.42. Mean ± SEM, repeated measures ANOVA with Bonferroni multiple comparison test. See also . BAC, bacterial artificial chromosome; C, clockwise; CC, counterclockwise; NP, no preference; SEM, standard error of the mean.

Article Snippet: Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).

Techniques: Control, Mutagenesis, Comparison

Journal: PLoS Biology

Article Title: Early uneven ear input induces long-lasting differences in left–right motor function

doi: 10.1371/journal.pbio.2002988

Figure Lengend Snippet: (A) Illustration of the contralateral and ipsilateral hemispheres regarding turning direction. (B) fEPSPs were greater ( p = 0.017) on the ipsilateral compared to contralateral side in Slc12a2 K842 * /K842 * mice. Inset: standard cortico-striatal fEPSP recording (black) and the sensitivity of the EPSP component to NBQX, an AMPA receptor antagonist (red); NP1: presynaptic fiber volley. fEPSPs between left and right hemispheres of littermate controls ( p = 0.94) or of mutants ( p = 0.96). Numbers in parentheses: number of recordings, number of mice. Mean ± SEM, two-way repeated measures ANOVA. See also . AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid; C, clockwise; CC, counterclockwise; EPSP, excitatory postsynaptic potential; fEPSP, field excitatory postsynaptic potential; NP1, negative peak 1; SEM, standard error of the mean.

Article Snippet: Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).

Techniques:

Journal: PLoS Biology

Article Title: Early uneven ear input induces long-lasting differences in left–right motor function

doi: 10.1371/journal.pbio.2002988

Figure Lengend Snippet: Western blot analysis and quantification indicate that ERK phosphorylation is up-regulated in the striatal hemisphere contralateral to the preferred direction of signaling in Foxg1 Cre/+ ; Slc12a2 fx/fx (contralateral versus ipsilateral, p = 0.027, n = 6 for mutants and n = 6 for controls) and BAC 316.23 mutants (contralateral versus ipsilateral, p = 0.045, n = 5 for mutants and n = 3 for controls). No significant differences in unphosphorylated ERK were detected between hemispheres in all comparisons. Mean ± SEM, two-tailed t-test. See also . BAC, bacterial artificial chromosome; ERK, extracellular signal-regulated kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; SEM, standard error of the mean.

Article Snippet: Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).

Techniques: Western Blot, Phospho-proteomics, Two Tailed Test

Journal: PLoS Biology

Article Title: Early uneven ear input induces long-lasting differences in left–right motor function

doi: 10.1371/journal.pbio.2002988

Figure Lengend Snippet: (A) Illustration of the hypothesized dependence of the preferred turning direction on asymmetric striatal p-ERK levels and how SL327 inhibition could reduce or reverse the directional preference; C, CC. (B) SLC327-inhibition of p-ERK in the contralateral ventral striatum and vehicle in the ipsilateral side of Slc12a2 K842 * /K842 * mutants reduces clockwise circling 1 day post injection ( n = 4; p = 0.0007 with two-tailed Chi-square and Fisher exact tests), while bilateral vehicle has no effect ( n = 7). SL327 injected ipsilaterally trended toward increasing the already high clockwise circling 1 day post injection ( n = 7). Repeated measures ANOVA with the Tukey multiple comparison test; mean ± SEM. See also . (C) PD0325901 inhibition of p-ERK in the contralateral ventral striatum and vehicle in the ipsilateral side of Slc12a2 K842 * /K842 * mutants also reduces clockwise circling 1 day post injection ( n = 8; p < 0.0001 with two-tailed Chi-square and Fisher exact tests), while bilateral vehicle has no effect ( n = 8). PD0325901 injected ipsilaterally, as with SL327, trended toward increasing the already high clockwise circling 1 day post injection ( n = 8). Repeated measures ANOVA with the Tukey multiple comparison test; mean ± SEM. See also . C, clockwise; CC, counterclockwise; ERK, extracellular signal-regulated kinase; MEK, mitogen activated protein kinase kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; SEM, standard error of the mean.

Article Snippet: Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).

Techniques: Inhibition, Injection, Two Tailed Test, Comparison

Journal: PLoS Biology

Article Title: Early uneven ear input induces long-lasting differences in left–right motor function

doi: 10.1371/journal.pbio.2002988

Figure Lengend Snippet: (A) Immunohistochemical staining of inner ear sections showed reduced SLC12A2 expression (brown) in Tbx1 Cre/+ ; Slc12a2 fx/fx mutants (Nissl counterstain, purple) and defects of the cochlea (top panel) and vestibular structures: saccular vestibular membrane collapse (middle) and ut and cr degeneration (bottom). (B) ABR testing revealed no waveforms at 100 decibels, sound pressure level, in 6-week-old mutants, indicative of deafness. (C) VsEP (P1, first peak; P2, second peak) were absent in mutants, indicative of vestibular impairment. (D) Mutants performed poorly on the rotarod test ( p < 0.0001), consistent with impaired balance. Mean ± SEM, n = 8 mice/genotype, two-tailed t test. See also . (E) Open field recordings of Tbx1 Cre/+ ; Slc12a2 fx/fx mutants showed a turning bias in either the C ( n = 8) or CC ( n = 10) direction (NP, n = 1). Mean ± SEM. See also . (F) Tbx1 Cre/+ ; Slc12a2 fx/fx mutants circle ( n = 37), whereas littermate controls ( n = 10) and Nestin-Cre;Slc12a2 fx/fx mutants do not ( n = 5). Mesp1 Cre/+ ; Slc12a2 fx/fx mutants, in which Slc12a2 expression is lost from the brain vasculature (Antoine et al., 2017), also do not circle. Mean ± SEM. See also . (G) Western blots of striatal lysates from Tbx1 Cre/+ ; Slc12a2 fx/fx mutants showed greater p-ERK1 in the contralateral relative to ipsilateral hemisphere, normalized to β-actin ( p = 0.046, n = 6). Asymmetries in p-ERK1 levels were not detected in Nestin-Cre;Slc12a2 fx/fx mutants ( n = 3). Mean ± SEM, two-tailed t test. See also . ABR, auditory brain stem response; C, clockwise; CC, counterclockwise; cr, cristae; NP, no preference; p-ERK, phosphorylated extracellular signal-regulated kinase; rm, Reissner’s membrane; SEM, standard error of the mean; SPL, sound pressure level; sv, stria vascularis; ut, utricle; vm, vestibular membrane; VsEP, vestibular sensory evoked potential.

Article Snippet: Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).

Techniques: Immunohistochemical staining, Staining, Expressing, Membrane, Two Tailed Test, Western Blot

Journal: PLoS Biology

Article Title: Early uneven ear input induces long-lasting differences in left–right motor function

doi: 10.1371/journal.pbio.2002988

Figure Lengend Snippet: (A) Open field recordings of Tbx1 Cre/+ ; Slc12a2 fx/fx mutants ( n = 10) before (day −1) and after unilateral surgery on the left ear showed a preference for CC turning by 14 days. See also . (B) Average CC and C turns for the mutants in panel A and littermate controls ( n = 10) with unilateral ear surgery. Two-way RM ANOVA with Tukey’s multiple comparison test (between mutant CC and C: at day 14, p = 0.0051; day 21, p = 0.0019; day 25, p < 0.0001). See also . (C) Activation during caloric vestibular stimulation of the right and left ear in a right- and left-hander with numbers of voxels exhibiting significant differences in effect size between left and right hemispheres (paired t test; n = 12 right-handers, n = 12 left-handers; p < = 0.001). See also . (D) Model of ear-induced lateralization. C, clockwise; CC, counterclockwise; fEPSP, field excitatory postsynaptic potential; p-ERK, phosphorylated extracellular signal-regulated kinase; RM, repeated measure.

Article Snippet: Primary antibodies include mouse NeuN (1:100, Millipore, Burlington, MA), rabbit GFAP (1:500, Dako, Santa Clara, CA), goat SLC12A2 (1:50, Santa Cruz Biotechnology, Dallas, TX), rabbit SLC12A2 (1;100, Proteintech Group, Rosemont, IL), rabbit Laminin (1:200, Millipore), rabbit GFP (1:100, Life Technologies, Carlsbad, CA), rabbit p-ERK (1:200, Cell Signaling, Danvers, MA), mouse Islet1 (1:2, Developmental Studies Hybridoma Bank, Iowa City, IA), and mouse Pou3f1/Oct6 (1:50, Millipore).

Techniques: Comparison, Mutagenesis, Activation Assay